Philips Designs the Light Blossom an Intelligent Street Light Concept

first_img Citation: Philips Designs the ‘Light Blossom,’ an Intelligent Street Light Concept (2008, October 17) retrieved 18 August 2019 from https://phys.org/news/2008-10-philips-blossom-intelligent-street-concept.html Explore further Designing nighttime lighting solutions for urban areas presents a challenge for city planners. Too much light results in light pollution – not just limiting the enjoyment of stargazers, but also interfering with the routines of plants and animals. On the other hand, having too little light threatens a neighborhood’s overall safety. The Light Blossom collects energy from the sun and wind during the day. At night, the device glows dimly when no one is around, and brighter when it senses motion, such as people walking nearby. Image: Pocket-Lint. This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only. Apple’s next iPhone crop must fight off more pests One solution to the urban lighting problem is a new concept called “Light Blossom,” designed by Philips Electronics. Light Blossom is an intelligent LED lighting system that can provide bright light when it senses people walking nearby, and decrease its luminosity when people aren’t around. The technology is also energy-efficient and operates off the grid, gathering solar and wind energy during the day to use for light at night. During the day, Light Blossom works similar to a flower, opening its “petals” to collect solar energy. As the sun moves across the sky, the petals gradually reorient themselves so they’re facing the sun head-on to operate at maximum efficiency, similar to a sunflower. On cloudy days when the wind is strong, the Light Blossom automatically converts its petals into an upward, open position that allows them to catch the wind. As the petals rotate, they transfer the motion to a built-in rotor that converts the motion to energy. The Light Blossom continuously switches between solar and wind modes depending on weather conditions. It also displays its energy-collecting flow on its “trunk,” or pole, with a decorative light for passers-by to see.When the sun sets, the Light Blossom’s LEDs automatically turn on, illuminating the ground below it. Philips claims that the downward-facing lamp design minimizes light pollution enough to enable people to see the stars in some areas. When people pass by the light, proximity sensors detect their movement and the LEDs switch from dim stand-by mode to a higher-intensity light. Philips says that the Light Blossom’s energy-efficient LEDs use just half of the energy of a traditional street light to produce the same light output. Because the device doesn´t require power infrastructure, rural communities without electricity could install Light Blossoms without investing in grid infrastructure. In urban communities, the devices could even supply power back to the grid when they generate an excess of energy, making the Light Blossom a light pole that generates rather than consumes power.Philips unveiled the Light Blossom concept earlier this week at its 2008 Philips Simplicity Event, held in Moscow, Russia.More information: Philips last_img read more

Energizer Duo battery charger hides a Trojan

first_img Citation: Energizer Duo battery charger hides a Trojan (2010, March 9) retrieved 18 August 2019 from https://phys.org/news/2010-03-energizer-duo-battery-charger-trojan.html © 2010 PhysOrg.com Energizer has now taken the software for the model CHUSB charger off the market and removed the site from which it could be downloaded, and the company is asking customers who downloaded the Windows version to uninstall it. There are easy steps to fight the Trojan in affected machines, and Macintosh users are not affected.Symantec’s Director of Global Intelligence, Dean Turner, said it’s impossible to be certain the Trojan has always been in the software that monitors the Duo USB charger, but the Trojan’s binary header states it was created in May 2007. It is not known how the Trojan came to be in the software, but malware has previously been found to be hidden inside products. Energizer is working with the US Computer Emergency Readiness Team (US-CERT) and the US government to try to find out how the code found its way into the software.The Trojan allows an attacker to operate with the same privileges as the user who is logged in, and to remotely control the system via connections on 7777/tcp to send and receive files, run programs, and list the contents of directories.US-CERT advises that to fix the problem, users can delete the Arucer.dll file from the Windows system32 directory, and then restart the system. An alternative fix is to remove the USB charger software. The Trojan Arucer.dll file will still be present but the code cannot be executed in the absence of the charger software. It is also advisable to block access to port 7777 using a firewall or via network perimeter devices.Energizer’s Duo USB battery chargers have been available in the US, Europe, Asia, and Latin America since 2007. They allow computer users to recharge the Nickel Metal Hydride (NiMH) batteries either from a wall outlet or a USB connection. It also enabled the user to monitor the status of charging on the PC. This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only. Explore furthercenter_img (PhysOrg.com) — The Energizer Duo USB battery charger has been hiding a backdoor Trojan in its software that affects computers using Windows. According to Symantec the Trojan has probably been there since 10th May 2007. Energizer Duo USB battery charger New trojan detected for Microsoft Wordlast_img read more

Scientists urge halt to road through Serengeti

first_img © 2010 PhysOrg.com Image credit: Nature, doi:10.1038/467272a A group of 27 scientists, reporting in the journal Nature yesterday, say the proposed two-lane 50 km road through the northern end of the park would curtail the migration of wildebeest, and according to their computer simulation, this would cause a collapse in what is the largest migratory system on Earth, and could lead to a drop in the wilderbeest population from 1.3 million to under 300,000. It would also affect the annual migration of 1.5 million zebras in the park, and have knock-on effects on the grasslands ecosystems.The road would be used by vehicles carrying goods, including herbicides, pesticides, and seeds of potentially invasive species. The scientists say it would be a source of chemical pollutants such as lead and other heavy metals that would flow into waterways in high concentrations in the rainy season. They say a road dividing the park would also make life easier for poachers, and would increase human conflicts with the animals in the park.The planned road is to form part of links between the coast of Tanzania and Uganda, Rwanda, Burundi, Lake Victoria, and the Democratic Republic of Congo, and there has been growing pressure to build a road to facilitate commerce and trade in the region.The scientists, led by Andrew Dobson of Princeton University, propose an alternative route running to the south of the park, which they say will bring greater benefits to the environment and development without threatening the wildebeest population or the tourist industry, which supports thousands of people. If built in the south the road would need to be longer, but would be less expensive, and would also serve almost five times as many people as the planned route through the north.Nature cautioned in an editorial that the current proposal is for a gravel road and the predictions are based on a fenced tarmac road, which the scientists say would probably follow. The journal also cautioned that criticisms from foreigners could have a negative backlash or cause entrenchment of attitudes. (PhysOrg.com) — Scientists have appealed for a halt to plans to build a road through the Serengeti National Park in Tanzania in 2012, saying it will be an environmental disaster. Zoological Society: Commercial road would disrupt world’s greatest migration Explore further Citation: Scientists urge halt to road through Serengeti (2010, September 17) retrieved 18 August 2019 from https://phys.org/news/2010-09-scientists-urge-halt-road-serengeti.html This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only. More information: Road will ruin Serengeti, Nature 467, 272-273 (16 September 2010) doi:10.1038/467272a ; Published online 15 September 2010last_img read more

Where does the Amazon start Study points to source

first_img Citation: Where does the Amazon start? Study points to source (2014, February 16) retrieved 18 August 2019 from https://phys.org/news/2014-02-amazon-source.html This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only. Explore further (Phys.org) —The Amazon is believed to be the world’s largest river. A tough question has been where that river actually begins. Naming its source has evidently been difficult as centuries of efforts indicate. With technology and scholarship on hand why should deciding on the true source be so difficult? Various sites in Peru have been pinned as the source through the years. A study published online this month in the journal Area makes the case for Amazon’s source to be the Mantaro River in southwestern Peru, even though the Apurímac River has been said since 1971 to be the source. Jane Lee of the National Geographic wrote on Thursday that, if accepted, the newer findings could affect measurements of the length of the Amazon River. That length is measured at about 4,000 miles by the U.S. Geological Survey. The discovery would add 47 to 57 miles to the Amazon’s length. New river dolphin species found in Brazil (Update) Solimões, the section of the upper Amazon River. Image: Wikipedia. More information: Correct placement of the most distant source of the Amazon River in the Mantaro River drainage, Area, Volume 46, Issue 1, pages 27–39, March 2014. DOI: 10.1111/area.12069AbstractThe Amazon River (‘Río de las Amazonas’) has the highest discharge of any river in the world and is also the longest or second longest. Its source has therefore intrigued scholars and explorers for centuries. A river’s source is often defined as the most distant upstream point in the drainage basin. For the past several decades, the longest upstream extension of the Amazon River has been held to be the Nevado Mismi area of the Río Apurímac drainage. We overturn this longstanding view by employing topographic maps, satellite imagery, digital hydrographic datasets and GPS tracking data to show that the Cordillera Rumi Cruz (10.7320°S, 76.6480°W; elevation ∼5220 m) in the Río Mantaro drainage lies 75–92 km further upstream than Nevado Mismi. We compare various methods for measuring each of the Río Apurímac and Río Mantaro lengths, and show that high-resolution satellite imagery and GPS tracking most closely follow the path of the water. Our results reposition the ‘most distant source’ of the Amazon to a more tropical location, change the uppermost ∼800 km of river to this point, and add 75–92 km to the river’s maximal length. © 2014 Phys.org Exploring the source of the Amazon in this recent effort made use of a variety of measurements including GPS tracking data and satellite images. Explorer and kayaker James “Rocky” Contos embarked on the effort to identify the source. The study in the journal Area, authored by Contos and anthropologist and archaeologist Nicholas Tripcevich, is titled “Correct placement of the most distant source of the Amazon River in the Mantaro River drainage.” They wrote that “Our results reposition the ‘most distant source’ of the Amazon to a more tropical location, change the uppermost ∼800 km of river to this point, and add 75–92 km to the river’s maximal length.”Contos, a kayaker, had said elsewhere in a biographical sketch: “In 2012 I discovered the true source of the Amazon, paddled all the headwaters of the Amazon and then completed the journey to the Atlantic, making the first complete descent of the Amazon River.” Why had the Mantaro been overlooked before this recent naming? The authors say that a bend or kink in the river’s lower half makes it look much shorter than it really is. Also, how do you define a river’s source? The definition affects the determination of the Amazon’s source, and there appears to be more than one definition. An historical definition of the “source” of a river is the point at which water must flow the greatest distance to flow into the river. Lee said that, according to the geographer at National Geographic, Juan Valdes, continuously flowing water is important in determining the true source of a river. Lee also said that the internationally accepted definition is the most distant point of a river’s longest tributary that flows continuously. The Mantaro River is said to run dry for about five months of the year. The Smithsonian National Air and Space Museum’s Andrew Johnston, geographer, considers the new study as providing a fresh perspective, according to Lee’s report in National Geographic. Assuming the measurements stand up to scrutiny, said Johnston, the Mantaro could be considered as a new source of the Amazon. Scientists, he added, should view three of proposed source tributaries—the Marañón,—throughout the 1700s, the Marañón River in northern Peru had the distinction of being the Amazon’s source—the Apurímac, and the Mantaro—in order to truly understand the Amazon.last_img read more

GraecoRoman papyrus memoirs reveal ancient Egyptian treatment for hangover

first_img Egypt archaeologists find pharaoh chapel More information: Graeco-Roman Memoirs 101 : www.ees-shop.com/index.php?mai … e63iettet1kgr770gnj3via Discovery Citation: Graeco-Roman papyrus memoirs reveal ancient Egyptian treatment for hangover (2015, April 24) retrieved 18 August 2019 from https://phys.org/news/2015-04-graeco-roman-papyrus-memoirs-reveal-ancient.html (Phys.org)—Researchers working on the Egypt Exploration Project, part of which is a task that involves translating thousands of documents nearly two thousand years old, written on sheets of papyrus, in Greek, into other languages, have published another manuscript, the 80th so far—this one centered on ancient medicinal treatments, one of which involves a “drunken headache cure.” The documents (now kept at Oxford’s Sackler Library) were found in a sand covered ancient trash heap outside the ancient town of Oxyrhynchus in Egypt back in 1898—researchers have been working ever since on translating them, periodically publishing a manuscript. The latest group offers historians a glimpse of how physicians attempted to aid the afflicted. Stringing together twigs from a shrub called Alexandrian laurel and then wearing it around the neck, for example, was prescribed for those that were feeling the pain of a night of bacchanalia. Thus far, it has not been determined if the treatment actually worked, but no doubt after the announcement of a cure, many who live where such a shrub grows will be testing its efficacy very soon. The Greeks and Romans used the plant to make head wreaths for athletes or others to mark them as important.Oxyrhynchus is approximately 100 miles south of Cairo, and was important in ancient times—it grew in size after Alexander the Great conquered the region, and maintained some degree of prominence during Roman and Byzantine times. After that, the population dwindled and the town fell into decline. The documents, collectively known as the Oxyrhynchus Papyri were discovered by Arthur Hunt and Bernard Grenfell, archeologists at Oxford—they were among other items that were part of a massive dumping ground—a sand covering helped to preserve the delicate papyrus sheets. Texts from the site span approximately five hundred years, from the first century AD to the sixth century.Other remedies listed on the 30 translated sheets included treatments for a toothache, hemorrhoids, and several that were aimed at relieving problems associated with various eye conditions—one excerpt even described a minor surgical procedure to repair a condition where an eyelid began to grow inside out.center_img © 2015 Phys.org Explore further This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.last_img read more

20millionyearold kangaroo relative found to be a hopper

first_img Phylogenetic timescale of Macropodoidea (white square) depicting the convergent appearance of arboreal/ scansorial locomotion (green) in balbarids and crown Macropodidae (black square). Background colour gradient infers the hypothesized locomotory transition from predominantly terrestrial quadrupedal progression (tan), as characterized by the extant quadruped Hypsiprymnodon moschatus (top right body-form silhouette), to increasingly bipedal saltating gaits (yellow) in crown potoroines and macropodines (bottom right body-form silhouette); this is correlated against a schematic of changing palaeohabitats through time (modified from Kear et al.). Dashed outline indicates uncertain taxonomic assignment of QM F59022 –QM F59025. Graphics produced with Adobe CC2016 by B.P.K. Credit: Royal Society Open Science (2019). doi/10.1098/rsos.181617 Explore further Credit: CC0 Public Domain A team of researchers with the Swedish Natural History Museum and Uppsala University has found evidence of hopping by a 20-million-year-old kangaroo relative. In their paper published in the journal Royal Society Open Science, the group describes their study of fossils unearthed in northwest Queensland, Australia, and what they found. © 2019 Science X Network More information: Climbing adaptations, locomotory disparity, and ecological convergence in ancient stem ‘kangaroos’ , Royal Society Open Science (2019). rsos.royalsocietypublishing.or … /10.1098/rsos.181617 Journal information: Royal Society Open Science Scientists have been puzzling over the history of kangaroo hopping for many years—because of the lack of kangaroo fossils older than 2 million years, though, it has been difficult to pinpoint when they evolved from walking or climbing creatures to animals that stand upright and get around by hopping. Prior research has suggested that the development of hopping was the result of a changing climate—Australia was once a lot wetter than it is today, with forests covering land that is now overrun with grasses. Standing upright in grasslands is advantageous, as can be seen in prairie dog behavior in parts of the U.S.; hopping is a way to move very quickly in such an environment, as observed with rabbits, most of which are also native to grasslands. In this new effort, the researchers have found evidence that suggests hopping might have started long before grasslands took over large parts of Australia, casting doubt on current theories about the evolutionary timeline of kangaroo hopping.The work by the researchers involved studying the fossil remains of an ancient marsupial species called balbarids—they were distant cousins of kangaroos and went extinct approximately 10 to 15 million years ago. Recently, one of the most complete balbarid skeletons ever found was unearthed, providing the opportunity to learn more about both the species and the origin of hopping in Australian creatures. The fossils were dated to approximately 20 million years ago. To better understand how the balbarid and other close relatives likely moved around, the team built models of them based on leg, ankle and other bone shapes. In so doing, they discovered that some of the creatures were likely climbers, others crawlers, and some walked upright and hopped around like modern kangaroos. The researchers suggest these findings mean that the history of kangaroo hopping will have to be revisited, because if ancient ancestors were hopping around 20 million years ago, it seems possible that kangaroos were, too. A new take on kangaroo evolution Citation: 20-million-year-old kangaroo relative found to be a hopper (2019, February 6) retrieved 18 August 2019 from https://phys.org/news/2019-02-million-year-old-kangaroo-relative-hopper.html This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.last_img read more

Microfluidics 2D fingerprints of heterogenous proteins in solution

first_img , Applied Physics Letters Microfluidic systems are used in molecular biology, biochemistry and biotechnology to rapidly analyze heterogenous biomolecular mixtures with high recovery rates and minute sample volumes. However, it is challenging to combine preparative and analytical processes within a single device for fast integrated analysis. In a recent study now published on Microsystems and Nanoengineering, Kadi L. Saar and co-workers at the interdisciplinary departments of chemistry, physics, and Fluidic Analytics Limited in Cambridge, U.K., have developed a chip combining the two steps of preparation and analysis. The device had the additional benefit of performing the entire process fully ingrained for electrophoretic separation in free solution, allowing researchers to obtain a quantitative map in a few minutes—much faster than conventional techniques. The mixture was unaffected by the supporting medium, and the researchers could study both weak and non-covalent molecular interactions. Saar et al. designed the device using a native phase quantitative electrophoresis unit connected to a microfluidic diffusional device sizing (MDS) unit. The combined platform allowed components of specific electrophoretic mobility (µe1) for on-chip downstream analysis, as a function of the applied electric field strength. They designed three channels downstream of the electrophoresis unit to maintain products of electrolysis away from the chip without entering the device. They minimized the number of individual units that drove the flow in the device, coupled to stable device operation for quantitative sample characterization. The scientists kept the outlets of the electrolyte separate from the combined device to apply electric potential across the device without generating an electrical short-circuit, and to allow the efficient removal of any electrolysis product without accumulation to prevent pressure fluctuations.The research team applied the electric potential on metallic connectors to generate a metal and fluid interface outside the chip in accordance with the device prototype designed by the same team. In this work, Saar et al. designed a Y-shaped flow splitter and kept the streams apart until they reached the splitter to prevent partial short circuiting. They calculated the flow rate of the electrolyte into the device to have an effect on device performance. , Nature Device design and operation. A free-flow electrophoresis unit (green), allowing rapid separation of analyte molecules in their native phase and yielding quantitative information on the separation process, directed fractions of the sample (yellow) to a downstream analysis process involving microfluidic diffusional sizing (MDS; pink). The sample was characterized by monitoring a single imaging frame (top right inset) to simultaneously extract the sizes and the charges of the separated fractions. The device was operated by applying a negative pressure at its outlet with a Y-shaped off-chip flow splitter keeping the solutions from the “low mobility waste” and “high mobility waste” channels separated to avoid partial short circuiting of the device. The electric potential was applied from the electrolyte solution outlets employing a flowing electrolyte solution as described previously13. The flow of the electrolyte solution, which included a fluorescent tracer for its visualization, was chosen such that the electrolyte would reach its outlet rather than be withdrawn into the separation chamber without reaching it (top left inset), yet it would leak into the chamber by a controlled distance, leaving sufficient space for deflecting the sample beam (bottom inset). Credit: Microsystems & Nanoengineering, doi: 10.1038/s41378-019-0072-3 © 2019 Science X Network Saar et al. used the strategy to obtain two-dimensional (2-D) characteristic maps of the protein mixture as a proof-of-concept. They extracted quantitative information from the separation step and related the applied potentials to the electrophoretic mobilities of the species to estimate the device efficacy. They recorded the current flowing in the system during normal operation and when the separation chamber was short-circuited to estimate the total electrical resistance of the device and the electrodes. The researchers calculated the electrophoretic mobility as the movement of a particle in an electric field for each of the fractions. Based on the experimental data, the constructed 2-D characteristic map included the effective charge (q) and the hydrodynamic radius (Rh) of the mixture. The resulting elementary charge units of the specific proteins agreed with estimated values elsewhere. They obtained the full two-dimensional map by only monitoring a single imaging frame for rapid in solution analysis. The analytical time of the microfluidics device from separation to diffusional sizing and imaging approximated 14 seconds. The scientists constructed the experimental 2-D map using only 3 µL of sample across seven minutes in total, orders of magnitude faster than the time-scale to perform conventional 2-D protein gels. The research team conducted a wide range of biomolecular interactions, in solution, directly under native conditions that were previously challenging to perform in the lab.In this way, Kadi L. Saar and co-workers developed a microfluidic device combining on-chip separation with direct on-chip analysis to replace the existing conventional microscale approaches. Using the device, they rapidly analyzed a binary mixture of proteins that could not be identified as individual components via existing solution sizing approaches. They constructed a 2-D characteristic map of the heterogenous mixture on a rapid time-scale to open the possibility of protein characterization in solution at an unprecedented time-resolution compared to existing biophysical techniques. On-chip separation and analysis of mixtures in liquid phase. By adjusting the effective voltage, ΔVeff, molecules of a specific electrophoretic mobility, μ = μel, and hence of a specific charge to hydrodynamic radius ratio, qRh, can be directed to the analysis area with those of smaller (μ < μel) and larger (μ > μel) mobility values directed to the waste collection channels. As the applied voltage can be varied, only a single analysis unit is required and the width of the separation chamber can be kept constrained, allowing the device to retain a high voltage efficiency and a fast processing speed. In the analysis area, the fractions are sized through microfluidic diffusional sizing (MDS) by monitoring their spatiotemporal motion under laminar flow conditions. Credit: Microsystems & Nanoengineering, doi: 10.1038/s41378-019-0072-3 Explore further Initially, they used voltage to separate protein molecules within a binary mixture of equally sized biomolecules indiscernible via conventional sizing or resolving techniques. Thereafter, the research team used the new device to obtain a 2-D fingerprint of a heterogenous protein mixture. The results will open new possibilities to acquire rapid multiparameter data on biomolecular systems on a short time-scale. Microfluidics techniques are attractive to analyze biological samples due to very low sample requirements and a high recovery rate. The platforms can provide unsurpassable analysis speed at the level of individual units of operation or provide several units with directly combined workflow, without sample transfer between the units. Such transfers occur through connectors or tubes and introduce dispersion to the sample, affecting the system’s performance. The workflow proposed herein can separate heterogenous mixtures to determine the components of interest and reduce complexities for further processing of the mixture for its purification.Researchers had previously introduced a variety of continuous flow-based molecular separation strategies at the micron scale, including free-flow electrophoresis, dielectrophoresis, magnetophoresis and acoustophoretic separation. Detection strategies such as laser- or LED-induced fluorescence (LIF), chemiluminescence or electrochemical approaches can be ingrained in parallel within such microfluidic separation platforms. Analytical information on the separated compounds can be achieved with offline strategies such as mass spectroscopy or SDS-PAGE, but the techniques can limit the processing speed in a single device, causing sample loss or contamination. 3-D-printed device detects biomarkers of preterm birth Rapid two-dimensional characterization of a mixture of bovine serum albumin and human lysozyme. The quantitative nature of the electrophoretic separation unit allowed the applied potential to be related to the electrophoretic mobility μel of each of the fractions. These data were used in combination with the extracted hydrodynamic radii (Rh) to estimate the effective charges (q) of the molecules in each of the fractions and to construct a two-dimensional q vs Rh map of the mixture over a few minutes timescale by monitoring the device only at a single imaging frame. Credit: Microsystems & Nanoengineering, doi: 10.1038/s41378-019-0072-3 This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only. Journal information: Analytical Chemistry (a) The voltage applied across the electrophoresis chamber was adjusted in linear steps to direct specific fractions to analysis. (b) The fractions were then sized by imaging the diffusional sizing unit consisting of four channels in which the extent of the diffusion of the analyte molecules into their surrounding carrier buffer was monitored. (c) The average size of the analytes molecules in each of the fractions were found by fitting the observed fluorescent profiles for different hydrodynamic radii (Rh) and minimizing the least mean square error between the fit (red dotted line) and the data (blue continuous line). (d) The fluorescence intensity in the analysis area varied depending on the concentration of the analytes in each of the fractions (top) and the sizes of the components in a binary mixture of lysozyme and bovine serum albumin were found to be Rh = 1.9 nm and Rh = 3.6 nm (bottom). (e) This binary mixture could not be characterized using sizing techniques that do not involve pre-fractionation steps, such as dynamic light scattering (DLS; light blue dotted line). Its sizing was possible using the device described in this work (dark blue continuous line). Credit: Microsystems & Nanoengineering, doi: 10.1038/s41378-019-0072-3 Citation: Microfluidics: 2-D fingerprints of heterogenous proteins in solution (2019, July 29) retrieved 18 August 2019 from https://phys.org/news/2019-07-microfluidics-d-fingerprints-heterogenous-proteins.html In total, the microfluidics platform with the separation unit directed a fraction of the fluid flow to an MDS unit, which the research team connected to analytical SEC-MALS to identify the fractions leaving the separation module. To analyze the data and obtain average molecular size for each fraction, they used a numerical solver predicting the generation of particle distributions. Saar et al. compared the predicted distributions with experimental data and extracted the hydrodynamic radii of the particles in each fraction. They imaged the nozzle at which the sample met the carrier medium as a reference point on particle movement. The scientists adjusted the diffusional sizing channel or flow rate to accurately size the analyte molecules, orders of magnitudes larger or smaller in size. Since they engineered the microfluidics platform using Poly(dimethylsiloxane) (PDMS), the scientists eliminated any autofluorescence in the setup before analyzing the image data. They then used the device to analyze a binary mixture of sample proteins; bovine serum albumin and human lysozyme. To preserve the native states of the protein molecules they imaged the label-free samples with a home-built UV-wavelength based microscope and quantified the intrinsic fluorescence of the sample. Saar et al. confirmed the ability to separate the mixture into its components by first applying a set of voltages to record the fluorescent profiles. They then recorded the electrophoretic mobilities of the proteins (µ e1) combined with the flow rate in the device to characterize most proteins and their complexes. The scientists altered the flow rate or the applied voltage to analyze biomolecules with diverse biophysical parameters. Using the platform, they rapidly characterized mixtures of nanoscale molecules, where individual analytes showed similar sizes but diverse electrophoretic properties. Based on the resulting histogram, the research team confirmed the presence of two distinct samples. Comparatively, in an off-chip conventional separation approach, the latter step required fractionation by sample transfer from one analytical tool to another via interconnected tubes, limiting device performance. The total protein concentration in the study approximated 100 µM and the scientists accurately detected the sensitivity limit to an approximate 100 nM, relative to the intrinsic fluorescence of protein fractions. For optically non-active compounds, Saar et al. suggest an alternative detection and characterization strategy such as dry mass sensing. Saar et al. therefore developed fully integrated separation and quantitative characterization of heterogenous biomolecular samples in a single microfluidic device to overcome the existing limits by directly coupling on-chip separation to on-chip analysis and molecular sizing. The design feature permitted the analysis of a specific fraction by adjusting the applied field strength. They designed the device to identify separated fractions similar to SEC-MALS (size-exclusion chromatography with multi angle light scattering) or LC (chip)-MS ((on-chip)-liquid chromatography-mass-spectrometry) methods. More information: Kadi L. Saar et al. Rapid two-dimensional characterisation of proteins in solution, Microsystems & Nanoengineering (2019). DOI: 10.1038/s41378-019-0072-3 George M. Whitesides. The origins and the future of microfluidics, Nature (2006). DOI: 10.1038/nature05058 Mathew H. Horrocks et al. Fast Flow Microfluidics and Single-Molecule Fluorescence for the Rapid Characterization of α-Synuclein Oligomers, Analytical Chemistry (2015). DOI: 10.1021/acs.analchem.5b01811 T. Müller et al. Dry-mass sensing for microfluidics, Applied Physics Letters (2014). DOI: 10.1063/1.4902131last_img read more

The foodfashion connect

first_imgWe started with the salads and the beet root salad was perfect, just as the chef had promised. They also have an excellent goat cheese salad with pine nuts but that is for food connoisseurs who know their ricotta from their stilton.Cavalli Cafe has a special pizza what is an absolute must try. The crust is wafer thin with a delicate topping off tomato, onion rings, garlic chips, bacon bits and an egg, sunny side up, placed right in the middle. If you thought pizza with egg yolk was a bad idea – you could not have been more mistaken. It is divine. Also Read – ‘Playing Jojo was emotionally exhausting’While talking to chef Kapil Sethi, we got instantly obsessed with the Chilli Caramel Basa and ordered it right away. And boy are we glad we did. The perfectly cooked fish with the wok tossed noodles and spinach packs the right punch of sweet and chilli. If you go to Cavalli Cafe and not order this – then consider your effort wasted.The Sweet Lobster Linguine was also quite delightful but the Chilli Basa had knocked us over. Try the hot chocolate and rum foam for dessert or the poached fig tart. For the hard-core sweet lovers – go for the second option.They also have a special, uncomplicated menu for the kids so they don’t have to struggle with the finer flavours. That menu includes the old-favourite Mac and Cheese. But for the food lovers who are not 6-years-old, the menu has better surprises  to offer. Try those!Cavalli also has a killer Happy Hour deal and a meal for two costs about Rs 2000 without taxes and alcohol. Quite a deal! Head over – we surely will be, again.last_img read more

Myth music and stage

first_imgThe ongoing Bharat Rang Mahotsav’s day two saw diverse plays, multi-lingual, non-verbal and some mythology based. Theatre lovers couldn’t have asked for more.The first play of the day was Pampa Bharatha enacted by Samudaya group from Karnataka, was in Kannad. The play begins with two university scholars visiting Pampa’s hometown, Dharmapuri, in search of evidence of his work in his old age, and their encounters with characters from the past, who have witnessed the events. The plot moves forward with Pampa rising from the stone inscription erected in his memory by his friend and the Samantha king, Arikesari, and he also meets his favorite character from the Mahabharata, Karna. Also Read – ‘Playing Jojo was emotionally exhausting’In the course of this journey one experiences the hard realities of caste-based politics in society up till now, from the injustice done to Karna because of his caste to the political conspiracy leading to the murder of Pampa,  carried out by the then business class, the Jains and the Brahmins.Another interesting play staged was Those Who Could Not Hear the Music which was created by the group Ranan from Kolkata. The performance piece was doubly inspired by the life, music and writings of and on Beethoven and Vikram Seth’s novel – Also Read – Leslie doing new comedy special with NetflixAn Equal Music.Two letters form the core of this performance. One written by Julia, the protagonist of An Equal Music, to her fellow musician and lover Michael; the other written by composer Ludwig Van Beethoven to his brothers. Both deal with the pain of inevitable and irretrievable loss as their writers come to terms with their growing deafness – a loss all the more poignant since it involves the one sense central to the world of music that both individuals – one from contemporary fiction and one from European musical history – inhabit. This combination of text, movement, Kathak and Beethoven’s music explores the many individual and collective, personal and epic textures arising out of this deep sense of loss. Beethoven’s music evokes all these and so much more of human experience with unbelievable insight, nuance, power and passion.Hindi play Anji by Ank group from Mumbai is inspired by Dr Vasant Deo’s Hindi translation of Tendulkar’s original Marathi comedy, Chi Saubhagyakanshini. Undaunted by the fact that she is getting on in years but is yet to find the right match, Anjali Sharma (Anji) sets out to search for her dream man herself. During this journey, she comes across several characters, situations and difficulties. An explosive climax persuades us to re-examine our value system, especially concerning women.Multi-lingual play Urubhangam by group Kasba Arghya, Kolkata was a six-hour long with four intervals.The Play was a narrative initiated by Bhasa’s text of Urubhangam but expanding further to Vyasa’s Mahabharata or that exists as a performance text in our folk performances, to locate how a power centre is formed and how it gets demolished, how the pillars of the power structure get broken like the thighs of Duryodhona.It starts from the reminiscences of the empire after the Bharat war and attempts to trace out how it all happened. Arghya’s Urubhangam is a theatre of Mahabharata, depicted through the body of an Indian actor adopting Indian acting methodology, also attempting to traverse the multicultural mythological India in its six hours journey. It is a theatre of physicality, if someone calls it a narrative then it is a physical narrative. The intervals are actually celebrated in an informal space outside the proscenium through folk performances or music to connect the tales of the tale.Lastly, a play titled The Woman Who Didn’t Want to Come Down to Earth was a solo performance by Gabrielle Neuhaus.  This non-verbal play was a mini-series which deals with the life of a woman who refuses to touch ground. Confronted with external threats and demands, she obeys neither rule nor gravity and reaches absurd and extreme situations.Each episode portrays with humour and surreal imagery the woman’s personality, as she follows with dogged termination of her own path.Starting with a comic-style indoors aerial trip through a living room in which furniture becomes both obstacle and support in her quest to avoid encroaching dust, the piece soon develops a darker mood. She tentatively begins to take charge of her new surroundings before finding a triumphant, yet fragile new existence.Originating partly from autobiographical material, The Woman Who Didn’t Want to Come Down to Earth depicts in crisp and succinct images the universal challenge of the individual to stay true to oneself.When: On till 19 Januarylast_img read more

Paying Homage to Khwaja Ahmed Abbas

first_imgDay two and three of the six-day long Urdu Drama Festival presented a touching solo act written by Khwaja Ahmed Abbas and a stage presentation of two of his stories, under the banner Abbas ke Do Rang, highlighting his versatility and his socialist leanings as a story teller.The 26th edition of Urdu Drama Festival hosted by the Delhi Government’s Department of Art, Culture & Languages in association with Urdu Academy is celebrating  the centenary year of two great writers Khwaja Ahmed Abbas and Begum Qudsia Zaidi. While Khwaja Ahmed Abbas was also a celebrated filmmaker and journalist, Qudsia Ziadi was a pioneer of Indian Theatre. Also Read – ‘Playing Jojo was emotionally exhausting’Angrezi ki Ghalatiyon Se Bhara Ek Khat, an Urdu adaptation of the English short story Letar Firaum a Child to Mahtama Gandhi, written by the legendary writer Abbas, was enacted by Pierrot’s Troupe theatre group, on Tuesday evening.Directed by M Sayeed Alam, the play unfolds with 12 year old Anwar Ali writing a letter to Mahatma Gandhi immediately after the latter’s assassination. The letter captures the collective guilt of a nation for the death of Bapu. The young Anwar’s letter is full of spelling and grammatical related mistakes. However, this poorly composed letter highlights the significance of Gandhi Ji’s vision of a united and peaceful nation. Also Read – Leslie doing new comedy special with NetflixUnder Abbas ke do rang the first story, My Son Is My Enemy, revolved around a lawyer who transforms from a revolutionary freedom fighter into a wealth and power chasing professional, and finds himself pitted against his idealist son, who still stands for his father’s lost ideals. The second presentation Ajanta is a story of two lovers who flee the brutal partition violence in Bombay and fed up with senseless killing all around find solace and peace in the artistic brilliance of the caves of Ajanta.   The first three days of the festival were dedicated to the plays of or on Abbas while the last three days will be dedicated to the plays scripted by Zaidi. On the fourth day of the festival (Thursday), Jaan Har, a play written by Begum Qudsia Zaidi and directed by Sheikh Khairuddin will be performed by the Living Theatre Group. The fifth day (Friday) Qudsia’s Khalid ki Khala, directed by Chander Shekhar Sharma will be performed by Mask Players Art Group.The festival will end on Saturday with Qudsia’s play Dhoban ko Kapre Diye, directed by Danish Iqbal and presented by the Social Pride Welfare Society.When: On till 18 October Where: Sri Ram Centrelast_img read more